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KMID : 1137020100210010029
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Journal of Gynecologic Oncology
2010 Volume.21 No. 1 p.29 ~ p.37
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Expression pattern of the class I homeobox genes in ovarian carcinoma
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Hong Jin-Hwa
Lee Jae-Kwan
Park Joong-Jean
Lee Nak-Woo
Lee Kyu-Wan
Na Jung-Yeol
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Abstract
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Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis.
Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries.
Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries.
Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.
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KEYWORD
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Homeobox gene, Ovarian neoplasms, Carcinogenesis
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